The chrysanthemum stunt viroid (CSVd) is a small, single-stranded, infectious RNA forming a circular secondary structure (354 bp), which belongs to the Pospiviroidae family and are known to be highly contagious. The effects on cultivated chrysanthemum are severe, including stunting of plant height, reduced flower size, and flower color bleaching. However, the expression of the symptoms depends on the chrysanthemum cultivars. Probably found in all countries where chrysanthemums are grown. There is no chemical that can control CSVd and spread occurs readily by mechanical means, including plant-to-plant contact, handling during cultivation and contaminated cutting tools. The most popular method for detection consists in a membrane hybridization which allow have results in 20 hour but this results are not reliable and can generated false positives.