Acanthamoeba is one of the most frequent protozoa found in nature. The life cycle of Acanthamoeba species involves two stages: an active form or trophozoite and a latent form known as a cyst. The amoebas of the Acanthamoeba genus have the ability to live as microorganisms of free life in nature and occasionally invade a host and live as parasites within it. The most common types of infections caused by Acanthamoeba spp. they include EAG, disseminated infections (cutaneous and nasopharyngeal) and keratitis.